| Abstract Detail
Population Genetics/Genomics Bhardhwaj, Krithjgnan S [1], Shrotri, Saket [2], Gowda, Vinita [3]. Population genetics of the polymorphic flowering species Curcuma caulina. Curcuma culina ( Zingiberaceae), is a mass flowering, perennial rhizomatous plant with tall leafy stems (50 to 120cm) endemic to the state of Maharashtra. It is found in moist deciduous grassland habitat of the laterite plateaus in the Northern western ghats at an altitude range of 1000m to 1400m above sea-level. The flowers of C. caulina are polymorphic, with the flower color being either white or yellow and their lateral bracts varying in color from greenish-white (GW) to redish-white (RW).A prior study of the C. caulina population in the Kaas Plateau from our lab (TrEE lab, IISER Bhopal), divided this continuous color variation of the bracts into 3 categories based on the observed trimodal distribution and categorized individuals into 3 different morphs named Green-White(GW), Green-Red(GR) and Red-White(RW). In order to understand the population structure of these flower morphs we ask i) if the population structure gets grouped according to the morph and/or the geographic location. ii) are individuals from the same morph/location more related to each other than to individuals from other morphs/locations.
For the above mentioned questions , individuals have been sampled from three laterite plateaus in the northern Western Ghats of Maharashtra (from N to S): Raireshwar (RAI), Baghdad Point (BP), and Kaas Plateau (KAS). Individuals have also been sampled along the road connecting KAS and BP, and is referred to as KMR. All populations are polymorphic except the population of Raireshwar, which had the GW morph with yellow flowers unlike the white flowers found elsewhere. To answer our questions, double digest Restriction Site Associated (ddRAD) Sequencing was used. STACKS[Catchen et.al 2013] was used to assemble the loci and genotype individuals, and STRUCTURE (Prichard et al. 2000) was used to understand the population structure.The observed and expected heterozygosity values indicate that there isn't any inbreeding at morph level nor is it present at a geographical level. Fixation index (pairwise Fst) values indicate that the genetic difference between the populations increases linearly with increasing geographic distance. The population structure also does not seem to be influenced by the different morphs or by the geographical location.
1 - IISER Bhopal, Biological Sciences, Indian Institute Of Science Education And Research Bhopal, Department Of Biological Sciences, Tropical Ecology And Evolution (TrEE) Lab, Room 303, AB3, IISER Bhopal, Bhauri Campus, Bhopal By Pass Road, Bhopal, MP, 462066, India, Bhoapl, Madhya Pradesh, 462066, India
2 - Indian Institute Of Science Education And Research Bhopal, Department Of Biological Sciences, Tropical Ecology And Evolution (TrEE) Lab, Room 303, AB3, IISER Bhopal, Bhauri Campus, Bhopal By Pass Road, Bhopal, MP, 462066, India
3 - IISER Bhopal, Room 223, AB3, Dept. Of Biological Sciences,, IISER- Bhopal, Bhopal Bypass Road, Bhauri,, Bhopal, MP, 462066, India
Keywords:
none specified
Presentation Type: Oral Paper
Number: PGG4002
Abstract ID:1000
Candidate for Awards:Margaret Menzel Award |
