| Abstract Detail
Conference Wide Whittall, Justen [1], Narbona, Eduardo [2], del Valle, Jose Carlos [3], Berardi, Andrea [4]. The Promises and Perils of Pigments Part2: Quantifying Flower Color Across Scales (Part 1 was presented at Botany 2021). Flower color is an evolutionary metamodel in biology – a model trait whose biochemical, developmental and genetic pathway is largely conserved across a diversity of lineages. Metamodels allow for an integrated perspective of the ecological and evolutionary opportunities and constraints underlying natural phenotypic variation. Studying traits like flower color leverages the phylogenetic replication of independent evolutionary changes across diverse branches of the tree of life. However, methods for quantifying plant pigments and flower colors in particular are diverse and rapidly changing. Choosing the most appropriate method often depends on the question at hand. In our second flower color workshop on color quantification, we will introduce a variety of approaches to quantifying plant pigments with an emphasis on flower color, anthocyanins and the flavonoids in general. We will demonstrate several tools for quantifying flower color in both field and laboratory settings. These approaches will include how to capture and analyze color using digital images, UV-Vis spectral reflectance, thin-section petal microscopy, biochemistry (plate reader absorbance & HPLC-DAD-MS) and RNA-Seq transcriptome data of petals. We will demonstrate how to apply a range of animal vision models to compare perceived color. Real time transcriptome analysis vignettes will be presented. In general, we will present a toolbox for quantifying color allowing participants to tailor their methods depending on their individual lines of investigation.
1 - Santa Clara University, 500 El Camino Real, 500 El Camino Real, Biology Department, Santa Clara, CA, 95053, United States
2 - Universidad Pablo De Olavide (CIF: Q9150016E), Biología Molecular E Ingeniería Bioquímica, Carretera De Utrera Km 1, Seville, 41013, Spain
3 - Universidad de Sevilla
4 - Harvard University Herbaria
Keywords:
none specified
Presentation Type: Workshop
Number: W21001
Abstract ID:19
Candidate for Awards:None |
