Abstract Detail

Comparative Genomics/Transcriptomics

Garza, Elyssa [1], Daundasekara, Kasuni [1], Pepper, Alan [2].

Identification of Ni tolerance and low Ca tolerance QTL in Caulanthus amplexicaulis.

Plants endemic to harsh soils, such as serpentine, must adapt tolerance mechanisms for survival of multiple stresses specific to that environment. High concentrations of nickel and low calcium are two stress-related characteristics of serpentine soil. The diploid plant genomes of Caulanthus amplexicaulis var. barbarae (serpentine endemic, n=14), C. amplexicaulis var. amplexicaulis (granite-living individual, n=14), and a set of recombinant inbred lines have been compared to identify loci contributing to Ni tolerance and low calcium tolerance. Biomass was collected from plants grown in control and artificial media containing either high Ni or low calcium. Marker data was developed using MSG (Multiplex Shotgun Genotyping) and Genotype Corrector, and then analyzed in ICIM and qGene. QTL underlying Ni tolerance were identified on chromosomes 2, 6, 7, and 12. QTL for low Ca can be found on chromosomes 1 and 3.

1 - Texas A&M University, 100 Butler Hall, 3258 TAMU, College Station, TX, 77843
2 - Texas A&M University, Department Of Biology, Texas A&M University, TAMU 3258, 214 Bsbe, College Station, TX, 77843, United States

Keywords:
Abiotic stress
metal
Nickel
genome
Brassicaceae
low calcium
Serpentine.

Presentation Type: Oral Paper
Number: CGT5001
Abstract ID:627
Candidate for Awards:Margaret Menzel Award