Abstract Detail

Functional Genetics/Genomics

Baldwin, Ethan [1], Leebens-Mack, Jim [2], Alabady, Magdy [3].

Increasing genomic coverage in GBS libraries with separate digests using multiple enzyme combinations.

Genotype by sequencing (GBS) is a cost-effective sequencing method that utilizes restriction enzyme (RE) digestion of genomic DNA to reduce genomic complexity. While this reduction of complexity is necessary to increase read depth at each locus, it can also exclude heterochromatic regions of a genome with low recombination rates that may include genes influencing functionally important phenotypic variation. Methyl-sensitive REs selectively target gene-rich, euchromatic regions of the genome, but can over-reduce the representation of large chromosomal regions, especially in large plant genomes. The over-reduction of heterochromatic regions could negatively impact characterization of Quantitative Trait Loci (QTLs). This is especially problematic in large, repetitive plant genomes, where a lack of enzyme cutting in heterochromatic regions severely reduces homogeneous spatial representation across the genome. We propose a modification to GBS that uses three separate digestion steps with unique combinations of REs. This method has the potential to increase genomic coverage in comparison to traditional GBS approaches, while still retaining the reduction of genome representation needed to ensure good read depth for QTLs.

1 - 750 Gaines School Rd, Apt G119, Athens, GA, 30605, United States
2 - University Of Georgia, Plant Biology, 2101 Miller Plant Sciences, Athens, GA, 30602, United States
3 - University of Georgia, Plant Biology

Keywords:
GBS
genotyping
QTL.

Presentation Type: Poster
Number: PCG006
Abstract ID:770
Candidate for Awards:Phytochemical Best Poster Award