Abstract Detail

Phylogenomics

Overson, Rick [1], Johnson, Matthew [2], Bechen, Lindsey [3], Kinosian, Sylvia [3], Douglas, Norman [4], Fant, Jeremie B [5], Hoch, Peter [6], Levin, Rachel [7], Moore, Michael [8], Raguso, Robert [9], Wagner, Warren [10], Skogen, Krissa [11], Wickett, Norman [12].

A phylogeny of the evening primrose family (Onagraceae) with a focus on tribe Onagreae using a target enrichment approach.

The evening primroses (Onagraceae) include 664 species (803 taxa) with a center of diversity in the Americas, especially western North America. Ongoing research in Onagraceae includes exploring striking variation in floral morphology, scent composition, and breeding system, as well as the role of these traits in driving diversity among plants and their interacting pollinators and herbivores. However, these efforts are limited by the lack of a comprehensive, well-resolved phylogeny. Previous phylogenetic studies based on a few loci strongly support the monophyly of the family and the sister relationship of the two largest tribes but fail to resolve several key relationships. We used a target enrichment approach to reconstruct the phylogeny of Onagraceae using 303 highly conserved, low-copy nuclear loci. We present a phylogeny for Onagraceae with 169 individuals representing 152 taxa sampled across the family. Additionally, we explored phylogenetic relationships within tribe Onagreae. This is the largest tribe in the family, comprising 13 genera and 265 species. We used 299 low-copy nuclear loci for 393 taxa, representing nearly all species and sub-species, within tribe Onagreae to investigate relationships at a finer phylogenetic scale. Deep splits within the family are strongly supported, whereas relationships among closely related genera and species are characterized by extensive conflict among individual gene trees. This phylogenetic resource will augment current research projects focused throughout the family in genomics, ecology, coevolutionary dynamics, and the evolution of characters driving diversification in the family.

1 - Arizona State University, Tempe, AZ, 85287, USA
2 - Texas Tech University, Biological Sciences, 2901 Main Street, Ms3131, Lubbock, TX, 79409, United States
3 - Negaunee Institute for Plant Conservation Science and Action, Chicago Botanic Garden, Glencoe, IL, 60022, USA
4 - University Of Florida, Biology, PO Box 118525, Gainesville, FL, 32611, United States
5 - Chicago Botanic Gardens, Plant Biology And Conservation , 1000 Lake Cook Rd, Glencoe, IL, 60022, United States
6 - Missouri Botan. Garden, 4344 Shaw Blvd., St. Louis, MO, 63110, United States
7 - Amherst College, Amherst, MA, 01002, USA
8 - Oberlin College, Department Of Biology, 119 Woodland St., Science Center K111, Oberlin, OH, 44074, United States
9 - Cornell University, Dept. Of Neurobiology And Behavior, 115 Eastwood Terrace, 215 Tower Road, Ithaca, NY, 14850, United States
10 - Smithsonian Institution, Department Of Botany, MRC-166, P. O. Box 37012, Washington, DC, 20013, United States
11 - Chicago Botanic Garden, Conservation Scientist, 1000 Lake Cook Road, Glencoe, IL, 60022, United States
12 - Chicago Botanic Garden, 1000 Lake Cook Rd., Glencoe, IL, 60022, United States

Keywords:
phylogenomics
HybSeq
Onagraceae
evening primrose
Target Enrichment
systematics
phylogenetics.

Presentation Type: Oral Paper
Number: PHYLO III009
Abstract ID:1036
Candidate for Awards:None