Abstract Detail



Crops and Wild Relatives

Montalvo-Olivo, Jorge [1], Valderrama-Pacho, Virgilio [2], Sanchez-Onofre, Arcadio [3], Ricaldi-Sarapura, Joseph [3], Arbizu, Carlos I. [4].

Genetic diversity and population structure of native potatoes from Huancavelica, Peru by SSR markers.

The Peruvian Andean region possesses an incalculable wealth of native potatoes that contribute to food security and constitutes a gene pool for potato improvement. To date, genetic studies on Peruvian native potatoes are very limited. We here employed 12 simple sequence repeats (SSRs) markers to determine the genetic diversity and population structure of 554 native potatoes cultivated from 3600 to 4500 meters above sea level from nine peasant communities of Huancavelica department in Peru. A total of 116 bands were manually scored, generating a 554 × 116 presence/absence matrix with an average of 9.66 alleles per locus. A dendrogram was generated using the UPGMA clustering algorithm, and, similar to the principal coordinate analysis (PCoA), they did not show a clear identification of clusters. STRUCTURE analysis clustered the native potatoes into 15 populations. However, the principal coordinate analysis did not show a clear separation. Genetic diversity estimation was conducted considering the 15 groups (populations) identified by STRUCTURE software. Values of genetic diversity (i.e. expected heterozigosity) ranged from 0.007 to 0.207. Shannon-Wiener´s index varied from 0.74 to 3.08, indicating low to high levels of genetic diversity. This is probably due to their clonal propagation, cultural aspects of farmers, and the presence of geographic limits that separate each population of native potatoes. AMOVA revealed the greatest variation between populations (52.83%) and indicated that variability within populations is 47.17%. Population divergence (Fst) between clusters 1 and 2 revealed the highest genetic difference (0.66), and the lowest Fst was observed between clusters 4 and 19 (0.09). To the best of our knowledge, this is the first investigation employing molecular markers in native potatoes from Huancavelica in Peru, and as such it is hoped that this helps to pave the way towards its modern genetic improvement and sustainable management. However, further studies are needed for a more accurate evaluation of genetic diversity together with phenotypic traits of potatoes.


1 - Universidad Nacional de Huancavelica, Departamento de Ciencias Agrarias, Av. Agricultura 319, Huancavelica, 09001, Peru
2 - Universidad Nacional de Huancavelica, Departamento de Ciencias Agrarias, Av. Agricultura 319, 09001, Peru
3 - Universidad Nacional de Huancavelica, Departamento de Ciencias Agrarias, Av. Agricultura 319, 09001, Peru
4 - Instituto Nacional de Innovación Agraria, Centro Experimental La Molina, Dirección de Desarrollo Tecnológico Agrario, Av. La Molina 1981, La Molina, Lima, Lima, 15024, Peru

Keywords:
SSR
germplasm
Andean region
Solanum.

Presentation Type: Poster
Number: PCW011
Abstract ID:1054
Candidate for Awards:None


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