Abstract Detail

Recent Topics Posters

Venkateshwaran, Muthu [1], Creanza, Joseph [2], Larsen, Garrett [2], Siragusa, Gregory [2], Lazar, Frank [2], Guddie, Jenna [2], Ané, Jean-Michel [3].

Functional characterization of genetic suppressors of symbiotic genes in the model legume Medicago truncatula.

Legumes establish a symbiotic relationship with nitrogen-fixing rhizobia. This results in the formation of root nodules where atmospheric nitrogen is fixed into a plant available form, reducing the demand for exogenous nitrogen fertilizer, thus paving way for sustainable agriculture. Several genetic studies using the model legume, Medicago truncatula, have identified the genetic components necessary for these associations. However, there are missing links in our understanding of the symbiotic signaling cascade that results in this symbiosis. We have optimized the protocol for Ethylene Methane Sulfonate (EMS) induced mutagenesis of Medicago truncatula seeds to generate mutant population to screen genetic suppressors. Screening of large mutagenized population enabled us to identify genetic suppressors of three well-established symbiotic genes, Does not Make Infections 1 (DMI1), DMI2 and DMI3, for functional characterization of their regulatory roles in legume-rhizobia symbiotic signaling.

1 - University of Wisconsin Platteville, School of Agriculture, 1 University Plaza, 212 Pioneer Tower, Platteville, WI, 53818, United States
2 - University of Wisconsin Platteville, 1 University Plaza, Platteville, WI, 53818, United States
3 - University of Wisconsin-Madison, Department of Bacteriology, Madison, WI, United States

Keywords:
legume-rhizobia symbiosis
symbiotic signaling
plant-microbe interactions.

Presentation Type: Recent Topics Poster
Number: PRT018
Abstract ID:1327
Candidate for Awards:None