Abstract Detail

Phylogenomics

Netherland, Michael [1], Hearn, David [2].

Cyto-nuclear Discordance in Adenia.

Contradicting phylogenies, produced from different loci, are prevalent in phylogenetic investigations. These discrepancies are also referred to as gene tree conflict, gene tree heterogeneity, and phylogenetic discordance. Biological sources of discordance include incomplete lineage sorting, gene duplication and loss, and hybridization and subsequent introgression. It is necessary to uncover and accommodate sources of discordance to produce robust phylogenies. The plant genus Adenia is a morphologically diverse group of approximately 100 species found in Africa, Madagascar, and Southeast Asia. Chloroplast and transcriptomes for 33 and 43 species, respectively, were sequenced and de novo assembled, producing 33 draft plastid genomes and 389 single-copy orthogroups. Plastid coding regions were annotated and extracted from the assembled plastid genomes. Super-matrix and consensus species tree phylogenetic inference methods were used to infer robustly-supported phylogenies for both datasets using only single-copy loci. Two strongly supported, conflicting nodes were found between trees made from different inference methods, for both data sets. Four nodes of strongly supported conflict were found between plastid and transcriptome trees, which we hypothesize resulted from hybridization, which is supported by the results of introgression inference analyses using the D-statistic (ABBA-BABA test) and HyDe. This study provides evidence for deep hybridization in Adenia and the first phylogenomic analyses of the genus.

1 - Towson University, 8000 York Road, Towson, MD, 21252, United States
2 - Towson University, Biology, 8000 York Road, Towson, MD, 21252, United States

Keywords:
phylogenomics
Species tree
cytonuclear discordance
introgression.

Presentation Type: Oral Paper
Number: PHYLO II004
Abstract ID:603
Candidate for Awards:None