Abstract Detail


Bacci, Lucas [1], Judd, Walter [2], Michelangeli, Fabian [3], Cellinese, Nico [4], Skean Jr, James [5], Dagallier, Léo-Paul [3], Majure, Lucas [6].

The implementation of NGS in Melastomataceae: untangling the recent and rapid diversification of the Miconia sect. Sagraeoides (Melastomataceae) in the Greater Antilles.

Melastomataceae constitute one of the major radiations within the angiosperms, composed of 5000-5600 known species ranging from herbs to trees, lianas and epiphytes and are primarily distributed in the Neotropics. The diversity of the family appears to be tied to the diverse habitats in which they are found, their associated pollinators, fruit type and dispersal agents. However, the drivers behind such a massive and rapid diversification have not been well explored, mostly because our existing phylogenetic datasets are not sufficiently comprehensive. Several family-wide phylogenetic studies have been conducted for Melastomataceae, contributing to a better understanding of relationships among clades within the family. These previous studies have been based mostly on few plastid or nuclear loci, and since 2004, those same loci have been used to add specific taxa into a broader phylogenetic context. Despite being useful to clarify many deeper relationships and clade delineations, this dataset still lacks resolution when dealing with species rich clades, especially those representing rapid and recent radiations. Recently, a plastome dataset with 16 species from different clades across the family was published, and a Melastomataceae-specific probe set for target capture of nearly 400 nuclear loci was developed, based on both Memecylon (Olisbioideae) and Tibouchina (Melastomataeae), two quite distant related clades. Besides mostly resolving species level relationships (in Tibouchina and Memecylon), this dataset also was proven effective for resolving species complexes. Even though these studies showed that the NGS datasets can improve the resolution of phylogenetic hypotheses in the family, the Melastomataceae probes kit still needs to be tested in clades outside Olisbioideae and Melastomataeae. Moreover, it is still unknown if the plastomes are variable enough to be implemented for rapid radiations within the family.In this context, we selected the Miconia sect. Sagraeoides (Miconieae) as a model to test the efficacy of both NGS datasets in reconstructing evolutionary relationships in Melastomataceae. The clade has 25 species of montane small trees and shrubs, with axillary inflorescences and small 4-merous flowers and is endemic to the Greater Antilles, with its center of diversity in the Massif de la Hotte in Haiti (11 spp.). It was recovered in a previous phylogenetic hypothesis based on ETS, ITS, accD and psbK, and was dated as relatively recent radiation (3.67 mya) in a broader Miconieae biogeographic study. It is considered a rapid radiation, and species level resolution was lacking in Sanger sequencing datasets. Thus, we will be discussing: 1. How effective is the Melastomataceae probe kit for resolving species relationships in the Miconia sect. Sagraeoides, as compared to existing Sanger datasets? 2. Is the plastome dataset effective at resolving species level relationships, and are phylogenetic inferences based on both probe set and plastome datasets congruent? 3. Which historical patterns and processes led to the current distribution and diversification of the Miconia sect. Sagraeoides in the Greater Antilles?

1 - University Of Florida, Florida Museum Of Natural History, Herbarium FLAS, 1659 Museum Rd, Gainesville, FL, 32611, United States
2 - University Of Florida, Department Of Biology, 220 Bartram Hall, PO Box 118525, Gainesville, FL, 32611, United States
3 - The New York Botanical Garden, Institute Of Systematic Botany, 2900 Southern Blvd., Bronx, NY, 10458, United States
4 - University Of Florida, FLORIDA MUSEUM OF NAT. HISTORY, 1659 Museum Rd., 354 Dickinson Hall, Gainesville, FL, 32611, United States
5 - Albion College, Biology Dept, 611 E Porter Street, Albion, MI, 49224, United States
6 - Florida Museum Of Natural History, Department Of Natural History, 1659 Museum Rd. , Gainesville, FL, 32611, United States

genome skimming
Target Enrichment.

Presentation Type: Oral Paper
Number: SYST II007
Abstract ID:620
Candidate for Awards:None

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