Abstract Detail

Phylogenomics

Odufuwa, Phebian [1], Smith, James (Jim) [1], Mansfield, Don [3], Feist, Mary Ann [1], Darrach, Mark [5].

Phylogenetic analysis of Perennial Endemic North American (PENA) Clade of Apiaceae.

The Perennial Endemic North American Clade of the plant family Apiaceae (PENA) consists of plants adapted to dynamic environments and difficult ecological conditions. The plants that make up PENA form one of the largest plant radiations in North America, consisting of ~230 putative taxa. However, despite the large numbers and importance to North American flora, their phylogenetic relationships are still unclear. Convergent evolution and homoplasy in morphological traits have also contributed to the taxonomic confusion within the group, rendering morphological studies insufficient to delimit genera or even species within PENA. Currently, PENA consists of 19 genera: Lomatium, (the largest genus), Cymopterus, Zizia, Polytaenia, Thaspium, Taenidia, Pseudocymopterus, Eurytaenia, Aletes, Harbouria, Musineon, Neoparrya, Oreonana, Oreoxis, Podistera, Pteryxia, Shoshonea, Tauschia, and Vesper. A previous semi-comprehensive phylogenetic work was able to identify four sub-clades within PENA. Despite an increased sampling of individuals in the Lomatium triternatum complex, Sanger data was unable to resolve some species boundaries. Recent work demonstrated the utility of Angiosperms353 NGS baits kit to improve, but not yet fully resolve, relationships within this species complex. Thus, the goal of our project is to use the Angiosperms353 baits kit to reconstruct the phylogenetic relationships of PENA and use it to understand the diversification patterns and processes of speciation. Our work recovered >300 genetic loci from every named taxon within PENA and established the monophyly of the clade with the exception of Eurytaenia. Our preliminary maximum likelihood phylogenetic analysis showed that the genera belonging to the Eastern sub-clade including Zizia, Polytaenia, and Thaspium are sister to the remainder of the clade. Poor support and resolution among other genera may reflect incomplete lineage sorting that can be resolved with coalescent methods.

1 -
2 - BOISE STATE UNIVERSITY, Department Of Biological Sciences, 1910 University Drive, Ms1515, Boise, ID, 83725, United States
3 - College of Idaho, Biology, 2112 Cleveland Boulevard, Caldwell, ID, 83605, United States
4 - University Of Wisconsin, Wisconsin State Herbarium, 430 Lincoln Dr., Madison, WI, 53706, United States
5 - 20762 Hemlock St. NE, Indianola, WA, 98342, United States

Keywords:
phylogenetics
species delimitation
Generic boundaries delineation
Angiosperms353
Geophyte
Lomatium
endemic flora.

Presentation Type: Oral Paper
Number: PHYLO III002
Abstract ID:824
Candidate for Awards:None