Abstract Detail

Phylogenomics

Hay, Nikolai [1], Bailey, C. Donovan [2], Mandakova, Terezie [3], Hendriks, Kasper [4], Pryer, Kathleen [5], Windham, Michael [6].

A Hyb-Seq phylogeny of Boechera (Brassicaceae) and related genera.

Boechera is a genus of over 100 recognized species that is most diverse in western North America but also occurs in eastern North America and the Russian Far East. Over the past two decades, this group has become a model system for biologists studying the genetics and evolution of apomixis, as well as researchers interested in ecological and evolutionary genomics. Although Boechera is estimated to be just 2.5 million years old, it has diversified to form 80+ sexual diploid taxa and hundreds of hybrids, making it one of the most rapid diversifications recorded among plants. This incredible rate of evolution, together with rampant hybridization, has played a significant role in the inability of previous investigators to produce a well-supported phylogeny of Boechera. Past attempts at a Boechera phylogeny included fewer than ten genes and were plagued by very short branch lengths along the backbone and major discordance between plastid and nuclear phylogenies. To fully realize the research potential of Boechera as a model genus, a well-resolved phylogeny is essential. To resolve relationships within the tribe Boechereae, we have taken a Hyb-Seq approach with targeted sampling of sexual diploids. Our sampling includes 106 collections spanning all recognized species groups in Boechera plus at least one representative for each of the other eight genera of Boechereae. We have inferred a maximum likelihood phylogeny combining data from two hybrid capture bait sets that cover ~1100 unique low copy loci. The resultant phylogeny strongly supports the recognition of two major clades within Boechereae. One of these encompasses nearly all species of western North American Boechera, while the other comprises all other genera of the tribe plus a monophyletic group of eastern North American and Far Eastern Boechera (Borodinia). These Hyb-Seq data also provide ample evidence of hybridization, both within and among major clades.

1 - Duke University, Biology, Campus Box 90338, Durham, NC, 27708, United States
2 - New Mexico State University, Biology, Rm 120 Foster Hall (Stores), Dept Of Biology - New Mexico State University, Las Cruces, NM, 88003, United States
3 - Masaryk University, CEITEC, Kamenice 753/5, Brno, Czech Republic
4 - Naturalis Biodiversity Center, PO Box 9517, 2300 RA , Leiden, Netherlands
5 - Duke University, Biology, Science Drive, Science Drive, Durham, NC, 27708, United States
6 - Duke University, Department Of Biology, Box 90338, Durham, NC, 27708, United States

Keywords:
Boechera
Hyb-Seq
phylogenetics
Brassicaceae.

Presentation Type: Oral Paper
Number: PHYLO III003
Abstract ID:840
Candidate for Awards:None